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Somatic cells with a heavy mitochondrial DNA mutational load render iPS cells with distinct differentiation defects.

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Author

Summary, in English

It has become increasingly clear that several age-associated pathologies associate with mutations in the mitochondrial genome. Experimental modeling of such events has revealed that acquisition of mitochondrial DNA (mtDNA) damage can impair respiratory function and, as a consequence, can lead to widespread decline in cellular function. This includes premature aging syndromes. By taking advantage of a mutator mouse model with an error-prone mtDNA polymerase, we here investigated the impact of an established mtDNA mutational load with regards to the generation, maintenance and differentiation of induced pluripotent stem (iPS) cells. We demonstrate that somatic cells with a heavy mtDNA mutation burden were amenable for reprogramming into iPS cells. However, mutator iPS cells displayed delayed proliferation kinetics and harbored extensive differentiation defects. While mutator iPS cells had normal ATP levels and glycolytic activity, the induction of differentiation coincided with drastic decreases in ATP production and a hyperactive glycolysis. These data demonstrate the differential requirements of mitochondrial integrity for pluripotent stem cell self-renewal versus differentiation, and highlight the relevance of assessing the mitochondrial genome when aiming to generate iPS cells with robust differentiation potential. Stem Cells 2014.

Publishing year

2014

Language

English

Pages

1173-1182

Publication/Series

Stem Cells

Volume

32

Issue

5

Document type

Journal article

Publisher

AlphaMed Press

Topic

  • Cell and Molecular Biology

Status

Published

Research group

  • Hematopoietic Stem Cell Development
  • Immunology

ISBN/ISSN/Other

  • ISSN: 1549-4918