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Evaluation of quantitative thin layer chromatography using staining reagents

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Author

Summary, in English

Thin layer chromatography (TLC) using staining reagents is a superior method for analyzing organic compounds without chromophores. It is fast, versatile and sometimes the only viable method. We have investigated quantitative TLC using staining reagents, in combination with modem image analysis software. Our results show that it is possible to get reliable measurements, suitable for high-throughput screening or physical organic investigations. The range of detection and the errors for the different parts of the process are illustrated. We show that the errors are largely due to the staining process and can be diminished by measuring ratios of compounds. (c) 2007 Elsevier B.V. All rights reserved.

Publishing year

2007

Language

English

Pages

298-305

Publication/Series

Journal of Chromatography A

Volume

1164

Issue

1-2

Document type

Journal article

Publisher

Elsevier

Topic

  • Organic Chemistry

Keywords

  • quantification
  • TLC
  • image analysis software
  • staining reagents

Status

Published

ISBN/ISSN/Other

  • ISSN: 0021-9673