U-2973, a novel B-cell line established from a patient with a mature B-cell leukemia displaying concurrent t(14;18) and MYC translocation to a non-IG gene partner.
Author
Summary, in English
B-cell lymphomas/leukemias with simultaneous t(14;18)(q32;q21) and MYC rearrangements have recently been shown to constitute a separate diagnostic entity, presenting with a rapid clinical course and a very poor prognosis. We describe the establishment of an Epstein-Barr virus negative cell line, designated U-2973, from a male patient with a de novo aggressive B-cell lymphoma/leukemia and very high peripheral blast cell count. Flow cytometry of bone marrow cells and U-2973 displayed a mature B-cell phenotype, and immunostaining showed expression of MYC and BCL2. IG gene rearrangement data were consistent with a lymphoid neoplasm of germinal centre derivation. Cytogenetic studies using conventional G-banding, fluorescent in situ hybridization, spectral karyotyping and single nucleotide polymorphism array demonstrated a complex karyotype with both a t(14;18) and double translocations between MYC and a non-IG gene partner located at chromosome 12p12.1.
Publishing year
2008
Language
English
Pages
218-225
Publication/Series
European Journal of Haematology
Volume
81
Issue
3
Links
Document type
Journal article
Publisher
Wiley-Blackwell
Topic
- Hematology
Keywords
- Oligonucleotide Array Sequence Analysis
- Molecular Sequence Data
- Male
- Lymphoma
- Leukocyte Count
- B-Cell
- Leukemia
- Karyotyping
- Fluorescence
- In Situ Hybridization
- Humans
- Immunoglobulin
- Genes
- Flow Cytometry
- Cytogenetic Analysis
- Pair 18
- Pair 14
- Pair 12
- Human
- Chromosomes
- Chromosome Aberrations
- Cell Line
- Bone Marrow Cells
- Adult
- Base Sequence
- Phenotype
- Polymorphism
- Single Nucleotide
- Proto-Oncogene Proteins c-bcl-2
- Proto-Oncogene Proteins c-myc
- Translocation
- Genetic
Status
Published
ISBN/ISSN/Other
- ISSN: 1600-0609