Molecular cloning of a small prostate protein, known as beta-microsemenoprotein, PSP94 or beta-inhibin, and demonstration of transcripts in non-genital tissues

Summary, in English

In order to study the gene expression of the seminal plasma protein beta-microseminoprotein, also known as PSP94 and beta-inhibin, clones encoding this protein were isolated from a cDNA library constructed in lambda gt11. Nucleotide sequencing confirmed the structure of a previously cloned cDNA. By northern blot analysis identical sized transcripts were demonstrated in the prostate, the respiratory (tracheal, bronchial and lung) tissues and the antrum part of the gastric mucosa. Thus, the protein is not primarily associated with male reproductive function. Although probably of no physiological significance, a slight structural similarity to the ovarian inhibin beta-chains was identified in the C-terminal half of the molecule.

• Author: M. Ulvsbäck; C. Lindstrom; Håkan Weiber; Per-Anders Abrahamsson; Hans Lilja; Åke Lundwall
• Department/s: Surgery; Department of Translational Medicine; Clinical Chemistry, Malmö
• Publishing year: 1989
• Language: English
• Pages: 5-1310
• Publication/Series: Biochem Biophys Res Commun
• Volume: 164
• Issue: 3
• Links: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=2590204
• Document type: Journal article
• Publisher: Elsevier
• Topic: Medicinal Chemistry; Surgery; Clinical Medicine
• Keywords: Prostate/*metabolism; Peptides/*genetics; Organ Specificity; Oligonucleotide Probes/chemical synthesis; Molecular Sequence Data; Male; Inhibins/genetics; Humans; Gene Library; DNA/*genetics; Comparative Study; Molecular; *Cloning; Northern; Blotting; Amino Acid Sequence; Base Sequence; *Prostatic Secretory Proteins; Proteins/*genetics; Research Support; Non-U.S. Gov't; Seminal Plasma Proteins; Sequence Homology; Nucleic Acid; *Transcription; Genetic
• Status: Published
• Research group: Surgery; Clinical Chemistry, Malmö