The browser you are using is not supported by this website. All versions of Internet Explorer are no longer supported, either by us or Microsoft (read more here: https://www.microsoft.com/en-us/microsoft-365/windows/end-of-ie-support).

Please use a modern browser to fully experience our website, such as the newest versions of Edge, Chrome, Firefox or Safari etc.

Identification of two juxtamembrane autophosphorylation sites in the PDGF beta-receptor; involvement in the interaction with Src family tyrosine kinases

På svenska

Author

  • Seijiro Mori
  • Lars Rönnstrand
  • Koutaro Yokote
  • Åke Engström
  • Sara A. Courtneidge
  • Lena Claesson-Welsh
  • Carl-Henrik Heldin

Summary, in English

Two novel sites of autophosphorylation were localized to the juxtamembrane segment of the human platelet-derived growth factor (PDGF) beta-receptor. To evaluate the importance of these phosphorylation sites, receptor mutants were made in which Tyr579, Tyr581 or both were replaced with phenylalanine residues; the receptor mutants were stably expressed in porcine aortic endothelial cells. Compared with the wild-type receptor, the Y579F and Y581F mutants were less able to mediate association with and activation of the Src family tyrosine kinases. The ability of these phosphorylation sites to mediate directly the binding of the Src family proteins was also demonstrated by using phosphotyrosine-containing synthetic peptides representing the juxtamembrane sequence of the receptor. Both the Y579F and Y581F mutants were similar to the wild-type receptor with regard to their protein tyrosine kinase activity and ability to induce mitogenicity in response to PDGF-BB. A conclusive evaluation of the role of the Src family members in signal transduction could, however, not be made since our attempt to prevent completely the association by mutation of both Tyr579 and Tyr581, resulted in loss of kinase activity and was therefore not informative. The present data, together with previous observations, demonstrate a high degree of specificity in the interaction between different autophosphorylation sites in the PDGF beta-receptor and downstream components in the signal transduction pathway.

Publishing year

1993

Language

English

Pages

2257-2264

Publication/Series

EMBO Journal

Volume

12

Issue

6

Document type

Journal article

Publisher

Oxford University Press

Topic

  • Medicinal Chemistry

Keywords

  • Amino Acid SequenceBase SequenceBinding SitesCell LineHumansMolecular Sequence DataOligodeoxyribonucleotidesPhosphorylationProtein-Tyrosine Kinases/*metabolismProto-Oncogene Proteins pp60(c-src)/*metabolismReceptors
  • Platelet-Derived Growth Factor/*metabolismSequence Homology
  • Amino Acid

Status

Published

ISBN/ISSN/Other

  • ISSN: 1460-2075