A metal binding site in the catalytic subunit of anaerobic ribonucleotide reductase.
Author
Summary, in English
A Zn(Cys)4 center has been found in the C-terminal region of the crystal structure of the anaerobic class III ribonucleotide reductase (RNR) from bacteriophage T4. The metal center is structurally related to the zinc ribbon motif and to rubredoxin and rubrerythrin. Mutant enzymes of the homologous RNR from Escherichia coli, in which the coordinating cysteines, conserved in almost all known class III RNR sequences, have been mutated into alanines, are shown to be inactive as the result of their inability to generate the catalytically essential glycyl radical. The possible roles of the metal center are discussed in relationship to the currently proposed reaction mechanism for generation of the glycyl radical in class III RNRs.
Department/s
Publishing year
2003
Language
English
Pages
3826-3831
Publication/Series
Proceedings of the National Academy of Sciences
Volume
100
Issue
7
Document type
Journal article
Publisher
National Academy of Sciences
Topic
- Biological Sciences
Status
Published
ISBN/ISSN/Other
- ISSN: 1091-6490