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NT-proBNP on Cobas h 232 in point-of-care testing: Performance in the primary health care versus in the hospital laboratory

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Author

  • Charlotte Gils
  • Ramshanker Ramanathan
  • Torben Breindahl
  • Mette Brokner
  • Anne L. Christiansen
  • Oyvin Eng
  • Ida J. Hammer
  • Catherine B. Herrera
  • Aina Jansen
  • Eva C. Langsjoen
  • Elisabeth S. Lokkebo
  • Toril Osestad
  • Anne D. Schroder
  • Lisa Walther
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Summary, in English

Background. NT-proBNP may be useful for ruling out heart failure in primary health care. In this study we examined the analytical quality of NT-proBNP in primary health care on the Cobas h 232 point-of-care instrument compared with measurements performed in a hospital laboratory. Materials and methods. Blood samples requested for NT-proBNP were collected in primary health care (n = 95) and in a hospital laboratory (n = 107). NT-proBNP was measured on-site on Cobas h 232 instruments both in primary health care centres and at the hospital laboratory and all samples were also analyzed with a comparison method at the hospital. Precision, trueness, accuracy, and lot-variation were determined at different concentration levels and evaluated according to acceptance criteria. Furthermore user-friendliness was assessed by questionnaires. Results. For Cobas h 232 repeatability CV was 8.5-10.7% in the hospital setting and 5.3-10.0% in the primary health care and within the analytical quality specifications, but higher than with the comparison method (<4%). NT-proBNP results obtained in primary health care were significantly higher than by the hospital comparison method (bias ranged from 14.3-23.7%), whereas there was no significant bias when Cobas h 232 was used in the hospital setting (bias ranged from 4.9 to 7.0%). User-friendliness of Cobas h 232 was overall acceptable. Conclusion. Cobas h 232 point-of-care instrument for measurement of NT-proBNP performed satisfactorily with regard to precision, user-friendliness, and lot-variation. A decrease in NT-proBNP levels observed in samples transported to a central laboratory needs further attention and investigation.

Publishing year

2015

Language

English

Pages

602-609

Publication/Series

Scandinavian Journal of Clinical & Laboratory Investigation

Volume

75

Issue

7

Document type

Journal article

Publisher

Informa Healthcare

Topic

  • Health Care Service and Management, Health Policy and Services and Health Economy
  • Cardiac and Cardiovascular Systems

Keywords

  • Natriuretic peptide
  • biological markers
  • heart disease
  • point-of-care
  • systems
  • validation studies

Status

Published

ISBN/ISSN/Other

  • ISSN: 1502-7686