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Bacterial glycosidases for the production of universal red blood cells

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Author

  • Qiyong P. Liu
  • Gerlind Sulzenbacher
  • Huaiping Yuan
  • Eric P. Bennett
  • Greg Pietz
  • Kristen Saunders
  • Jean Spence
  • Edward Nudelman
  • Steven B. Levery
  • Thayer White
  • John M. Neveu
  • William S. Lane
  • Yves Bourne
  • Martin L Olsson
  • Bernard Henrissat
  • Henrik Clausen
Show all

Summary, in English

Enzymatic removal of blood group ABO antigens to develop universal red blood cells ( RBCs) was a pioneering vision originally proposed more than 25 years ago. Although the feasibility of this approach was demonstrated in clinical trials for group B RBCs, a major obstacle in translating this technology to clinical practice has been the lack of efficient glycosidase enzymes. Here we report two bacterial glycosidase gene families that provide enzymes capable of efficient removal of A and B antigens at neutral pH with low consumption of recombinant enzymes. The crystal structure of a member of the alpha-N-acetylgalactosaminidase family reveals an unusual catalytic mechanism involving NAD(+). The enzymatic conversion processes we describe hold promise for achieving the goal of producing universal RBCs, which would improve the blood supply while enhancing the safety of clinical transfusions.

Publishing year

2007

Language

English

Pages

454-464

Publication/Series

Nature Biotechnology

Volume

25

Issue

4

Document type

Journal article

Publisher

Nature Publishing Group

Topic

  • Hematology

Status

Published

Research group

  • Transfusion Medicine

ISBN/ISSN/Other

  • ISSN: 1546-1696