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Neutrophils from vasculitis patients exhibit an increased propensity for activation by anti-neutrophil cytoplasmic antibodies.

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Author

Summary, in English

Anti-neutrophil cytoplasmic antibodies (ANCA) are thought to be pathogenic in ANCA associated vasculitis (AAV) by stimulating polymorphonuclear leukocytes (PMNs) to degranulate and produce reactive oxygen species (ROS). The aim of this study was to investigate if PMNs from AAV patients are more readily stimulated by ANCA compared with PMNs from healthy controls (HCs). Differences in ANCA characteristics that can account for different stimulation potential were also studied. PMNs from 5 AAV patients and 5 HCs were stimulated with 10 different IgGs, purified from PR3-ANCA positive patients, and ROS production, degranulation and neutrophil extracellular trap (NET) formation was measured. ANCA levels, affinity, and clinical data of the AAV donors were recorded. The results show that PMNs from AAV patients produce more intracellular ROS (p=0.019), but degranulate to a similar extent as PMNs from HCs. ROS production correlated with NET formation. Factors that may influence the ability of ANCA to activate PMNs include affinity and specificity for N-terminal epitopes. In conclusion, our results indicate that PMNs from AAV patients in remission behave quite similar to HC PMNs, with the exception of a greater intracellular ROS production. This could contribute to more extensive NET formation and thus an increased exposure of the ANCA autoantigens to the immune system.

Publishing year

2014

Language

English

Pages

363-372

Publication/Series

Clinical and Experimental Immunology

Volume

176

Issue

3

Document type

Journal article

Publisher

British Society for Immunology

Topic

  • Immunology in the medical area

Status

Published

Research group

  • Autoimmunity and kidney diseases

ISBN/ISSN/Other

  • ISSN: 0009-9104