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Sodium Butyrate Enhancement of Protein Expression

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Author

  • C Bassens
  • P Rigaux
  • V Hendrick
  • M Cherlet
  • K Sato
  • Knut Kotarsky
  • J Werenne

Summary, in English

We studied in chimeric EGFP-Photinus luciferase HeLa cells the expression of fluorescence and luminescence in different cell culture conditions (medium with or without Serum, static or dynamic conditions, in suspension or on microcarriers) as a model to follow the productivity of a recombinant protein. By studying the effect of Butyrate known as general but unpredictable stimulating agent for protein expression, we were able to establish the optimal conditions for efficacy ( concentration, time of addition, ...)

Department/s

  • Immunology

Publishing year

2005

Language

English

Pages

625-627

Publication/Series

Animal Cell Technology Meets Genomics

Volume

2

Document type

Book chapter

Publisher

Springer

Topic

  • Immunology in the medical area

Status

Published

Research group

  • Immunology

ISBN/ISSN/Other

  • ISBN: 978-1-4020-2791-8