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Mouse and rat cystatin C: Escherichia coli production, characterization and tissue distribution

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Author

Summary, in English

Recombinant mouse (Mus musculus) and rat (Rattus norvegicus) cystatin C were produced by expression in Escherichia coli, isolated and functionally characterized. The mouse and rat inhibitors were both fully active in titrations of papain. Determination of equilibrium constants for dissociation (Ki) for their complexes with the target proteinase, cathepsin B, produced values not largely different from that for human cystatin C (Ki 0.07-0.13 nM). Rabbit antisera against mouse and rat cystatin C were produced and used for improved affinity purification of the recombinant inhibitors. Affinity purified immunoglobulins isolated from the antiserum against mouse cystatin C were used for construction of a sensitive enzyme-linked immunosorbent assay. The assay was used to demonstrate a high degree of immunological cross-reactivity between mouse and rat cystatin C and could be used for cystatin C quantification in mouse and rat tissue homogenates. All tissues analyzed contained cystatin C, with a relative content very similar to that of human tissues. For all species, brain tissue contained the highest cystatin C amounts and liver the lowest, whereas kidney, spleen and muscle tissues were intermediate in content. In the mouse, a notable high cystatin C content in parotid gland tissue was observed. The high degree of similarity in distribution pattern and functional properties for mouse, rat and human cystatin C indicates that a murine model should be relevant for studies of the human disease, hereditary cystatin C amyloid angiopathy.

Publishing year

1996

Language

English

Pages

303-311

Publication/Series

Comparative Biochemistry and Physiology - Part B: Biochemistry & Molecular Biology

Volume

114

Issue

3

Document type

Journal article

Publisher

Pergamon Press Ltd.

Topic

  • Hematology
  • Medicinal Chemistry
  • Pharmacology and Toxicology

Keywords

  • Cathepsin B
  • cysteine proteinase inhibitor
  • cysteine proteinases
  • E. coli expression
  • enzymelinkedimmunosorbent assay
  • hereditary cystatin C amyloid angiopathy
  • amyloidosis

Status

Published

ISBN/ISSN/Other

  • ISSN: 1879-1107