A comparison of peripheral nerve regeneration in acellular muscle and nerve autografts
Author
Summary, in English
Regeneration of the rat sciatic nerve through acellular muscle and nerve autografts was evaluated 6-28 days postoperatively by the sensory pinch test, immunocytochemical staining for neurofilaments, and light and electron microscopy. Data points generated by the pinch test were plotted against postoperative time periods and by the use of regression analysis the initial delay period for muscle grafts was determined to 10.3 days. This value was similar to that previously published for acellular nerve grafts (9.5 days), but significantly longer than that for fresh nerve grafts (3.6 days). The calculated regeneration rate (slope of the regression line) for muscle grafts (1.8 mm/day) did not differ significantly ( p >0.05) from that calculated for acellular nerve grafts (2.1 mm/day) or for fresh nerve grafts (1.5 mm/day). The front of regenerating axons shown by axonal neurofilament staining confirmed the pinch test results. Both types of acellular grafts were repopulated with host non-neuronal cells and the muscle graft contained occasional ectopic muscle fibres. Remnants of graft basal laminae were evident at the ultrastructural level. These results indicate the suitability of either acellular muscle or nerve grafts for nerve repair despite their prolonged initial delay periods compared with conventional fresh nerve grafts.
Department/s
- Neural Interfaces
- Hand Surgery, Malmö
Publishing year
2003
Language
English
Pages
193-200
Publication/Series
Scandinavian Journal of Plastic and Reconstructive Surgery and Hand Surgery
Volume
37
Issue
4
Document type
Journal article
Publisher
Taylor & Francis
Topic
- Clinical Medicine
- Surgery
Keywords
- acellular
- regeneration
- autografts
- nerve
- muscle
Status
Published
Research group
- Neural Interfaces
- Hand Surgery, Malmö
ISBN/ISSN/Other
- ISSN: 1651-2073