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Down-regulation of apolipoprotein M expression is mediated by phosphatidylinositol 3-kinase in HepG2 cells.

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Author

Summary, in English

Apolipoprotein M (apoM) is a novel apolipoprotein present mostly in high-density lipoprotein (HDL) in human plasma. In the present study, we demonstrate that insulin, insulin-like growth factor I (IGF-I), and IGF-I potential peptide (IGF-IPP) significantly inhibits apoM expression, in a dose- and a time-dependent manner, in the human hepatoma cell line, HepG2 cells. Insulin-induced down-regulation of apoM was blocked by AG1024 (a specific insulin receptor inhibitor) and LY294002 (a phosphatidylinositol 3-kinase (PI3K) inhibitor), which indicates that it is mediated via the activation of PI3K pathway. In contrast, PD98059 (a MAP kinase inhibitor) did not influence insulin-induced down-regulation of apoM expression, and activation of neither PPAR-alpha agonist (GW7647) nor PPAR-gamma agonist (GW1929) influences apoM expression in HepG2 cells, which indicates that regulation of apoM expression is not related to the activation of PPAR-alpha and PPAR-gamma in hepatic cells, whereas, both PPAR-Of and PPAR-gamma agonists could inhibit apoB expression. Moreover, in the present study, we demonstrated that PPAR beta/delta agonist (GW501516) could inhibit both apoM and apoB expression in the HepG2 cells. In conclusion, this study shows that apoM expression is regulated by PI3-kinase in HepG2-cells. (c) 2006 Elsevier B.V. All rights reserved.

Publishing year

2006

Language

English

Pages

256-260

Publication/Series

Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids

Volume

1761

Issue

2

Document type

Journal article

Publisher

Elsevier

Topic

  • Medicinal Chemistry
  • Other Clinical Medicine
  • Pharmacology and Toxicology

Keywords

  • line
  • insulin
  • PPARs
  • apolipoprotein M
  • phosphatidylinositol 3-kinase and HepG2 cell

Status

Published

ISBN/ISSN/Other

  • ISSN: 1388-1981