In Vivo Reprogramming of Striatal NG2 Glia into Functional Neurons that Integrate into Local Host Circuitry.

Summary, in English

The possibility of directly converting non-neuronal cells into neurons in situ in the brain would open therapeutic avenues aimed at repairing the brain after injury or degenerative disease. We have developed an adeno-associated virus (AAV)-based reporter system that allows selective GFP labeling of reprogrammed neurons. In this system, GFP is turned on only in reprogrammed neurons where it is stable and maintained for long time periods, allowing for histological and functional characterization of mature neurons. When combined with a modified rabies virus-based trans-synaptic tracing methodology, the system allows mapping of 3D circuitry integration into local and distal brain regions and shows that the newly reprogrammed neurons are integrated into host brain.

• Author: Olof Torper; Daniella Rylander; Maria J M Pereira; Shong Lau; Tiago Cardoso; Shane Grealish; Malin Parmar
• Department/s: Developmental and Regenerative Neurobiology; StemTherapy: National Initiative on Stem Cells for Regenerative Therapy; MultiPark: Multidisciplinary research focused on Parkinson´s disease
• Publishing year: 2015
• Language: English
• Pages: 474-481
• Publication/Series: Cell Reports
• Volume: 12
• Issue: 3
• Links: http://www.ncbi.nlm.nih.gov/pubmed/26166567?dopt=Abstract; http://dx.doi.org/10.1016/j.celrep.2015.06.040
• Document type: Journal article
• Publisher: Cell Press
• Topic: Neurosciences
• Status: Published
• Project: Cell reprogramming: a new method for generating functional neurons
• Research group: Developmental and Regenerative Neurobiology
• ISBN/ISSN/Other: ISSN: 2211-1247