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Lactobacilli express cell surface proteins which mediate binding of immobilized collagen and fibronectin

På svenska

Author

  • G Lorca
  • MI Torino
  • GF de Valdez
  • Åsa Ljungh

Summary, in English

Binding of immobilized collagen-I (Cn-I) and fibronectin (Fn) by Lactobacillus acidophilus CRL 639 depends on cell-surface proteins. Capsule formation during the stationary growth phase has a negative effect on adherence of Cn-I and Fn. However, cells from the exponential growth phase. which produce no capsule, exhibit maximal binding. Binding is sensitive to trypsin, proteinase K, pronase E, and heat. Gelatin and soluble Cn-I partially inhibit binding of Cn-I although various proteins, sugars and amino acids do not affect binding to Fn. These results indicate that protein-protein interactions mediate adhesion to extracellular matrix proteins. SDS-PAGE and Western blot analyses of surface proteins revealed that several proteins including the major 43-kDa protein of the S-layer are expressed. Monoclonal antibodies showed that Fn binds to a 15-kDa protein, while Cn-I binds to proteins of 45 and 58 kDa.

Publishing year

2002

Language

English

Pages

31-37

Publication/Series

FEMS Microbiology Letters

Volume

206

Issue

1

Document type

Journal article

Publisher

Oxford University Press

Topic

  • Microbiology in the medical area

Keywords

  • immunoblot
  • polysaccharide
  • fibronectin binding
  • Lactobacillus
  • collagen binding
  • protein-protein interaction

Status

Published

ISBN/ISSN/Other

  • ISSN: 1574-6968