The Tetraspanin CD9 Affords High-Purity Capture of All Murine Hematopoietic Stem Cells

Summary, in English

Prospective isolation is critical for understanding the cellular and molecular aspects of stem cell heterogeneity. Here, we identify the cell surface antigen CD9 as a positive marker that provides a simple alternative for hematopoietic stem cell isolation at high purity. Crucially, CD9 affords the capture of all hematopoietic stem cells in murine bone marrow in the absence of contaminating populations that lack authentic stem cell function. Using CD9 as a tool to subdivide hematopoietic stem-cell-containing populations, we provide evidence for heterogeneity at the cellular, functional, and molecular levels.

• Author: Göran Karlsson; Emma Rörby; Cristina Pina; Shamit Soneji; Kristian Reckzeh; Kenichi Miharada; Christine Karlsson; Yanping Guo; Cristina Fugazza; Rajeev Gupta; Joost H. A. Martens; Hendrik G. Stunnenberg; Stefan Karlsson; Tariq Enver
• Department/s: Division of Molecular Medicine and Gene Therapy; Stem Cell Metabolism; Division of Clinical Genetics; StemTherapy: National Initiative on Stem Cells for Regenerative Therapy
• Publishing year: 2013
• Language: English
• Pages: 642-648
• Publication/Series: Cell Reports
• Volume: 4
• Issue: 4
• Full text: - 2 MB; Download statistics
• Links: http://dx.doi.org/10.1016/j.celrep.2013.07.020
• Document type: Journal article
• Publisher: Cell Press
• Topic: Cell Biology
• Status: Published
• Research group: Stem Cell Metabolism
• ISBN/ISSN/Other: ISSN: 2211-1247