A novel method for downstream characterization of breast cancer circulating tumor cells following CellSearch isolation.

Summary, in English

Enumeration of circulating tumor cells (CTCs) obtained from minimally invasive blood samples has been well established as a valuable monitoring tool in metastatic and early breast cancer, as well as in several other cancer types. The gold standard technology for detecting CTCs in blood against a backdrop of millions of leukocytes is the FDA-approved CellSearch system (Janssen Diagnostics), which relies on EpCAM-based immunomagnetic separation. Secondary characterization of these cells could enable treatment selection based on specific targets in these cells, as well as providing a real time window into the metastatic process and offering unique insights into tumor heterogeneity. The objective of this study was to develop a method for downstream characterization of CTCs following isolation with the CellSearch system.

• Author: Henrik Frithiof; Charlotte Welinder; Anna-Maria Larsson; Lisa Rydén; Kristina Aaltonen
• Department/s: Breastcancer-genetics; Tumor microenvironment; Division of Translational Cancer Research; The Liquid Biopsy and Tumor Progression in Breast Cancer; Breast Cancer Surgery; Surgery (Lund); BioCARE: Biomarkers in Cancer Medicine improving Health Care, Education and Innovation
• Publishing year: 2015
• Language: English
• Publication/Series: Journal of Translational Medicine
• Volume: 13
• Issue: 1
• Full text: - 21 MB; Download statistics
• Links: http://www.ncbi.nlm.nih.gov/pubmed/25896421?dopt=Abstract; http://dx.doi.org/10.1186/s12967-015-0493-1
• Document type: Journal article
• Publisher: BioMed Central (BMC)
• Topic: Cancer and Oncology
• Status: Published
• Project: CTC-MBC, Circulating Tumor Cells in Metastatic Breast Cancer; Breast Cancer Surgery
• Research group: The Liquid Biopsy and Tumor Progression in Breast Cancer; Breast Cancer Surgery
• ISBN/ISSN/Other: ISSN: 1479-5876