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Solubility of endotoxins from Escherichia coli and Pseudomonas aeruginosa

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Author

Summary, in English

Background The Limulus amebocyte lysate (LAL) assay may underestimate endotoxins because only soluble endotoxins are determined. The solubility of endotoxins was, therefore, studied in two species of Gram-negative bacteria. Methods Cultures were grown in serum broth, cells were harvested by centrifugation and washed in physiological saline. Bacterial suspensions were either filtered through PTFE filters and air dried at room temperature, dried in polypropylene tubes at room temperature in a desiccator, or freeze dried. Samples were extracted with aqueous Tween 20. The soluble and insoluble fractions were dried, methanolysed, and hydroxy-fatty acid methyl esters were determined by gas chromatography (GC) as markers of endotoxins. Results The solubility of the endotoxins from Escherichia coli and Pseudomonas aeruginosa ranged from 9% to 83%. Species and drying conditions had substantial influence on the solubility. Conclusions Endotoxin exposure may be underestimated in environmental samples by the LAL test because a substantial fraction can be non-soluble and is not detected. (C) 2004 Wiley-Liss, Inc.

Publishing year

2004

Language

English

Pages

375-377

Publication/Series

American Journal of Industrial Medicine

Volume

46

Issue

4

Document type

Journal article

Publisher

John Wiley & Sons Inc.

Topic

  • Microbiology in the medical area

Keywords

  • accuracy
  • endotoxins
  • solubility
  • Limulus amebocyte lysate test

Status

Published

ISBN/ISSN/Other

  • ISSN: 0271-3586