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Lesion-dependent regulation of transgene expression in the rat brain using a human glial fibrillary acidic protein-lentiviral vector.

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Author

Summary, in English

The ability to regulate transgene expression will be crucial for development of gene therapy to the brain. The most commonly used systems are based on a transactivator in combination with a drug, e.g. the tetracycline-regulated system. Here we describe a different method of transgene regulation by the use of the human glial fibrillary acidic protein (GFAP) promoter. We constructed a lentiviral vector that directs transgene expression to astrocytes. Using toxin-induced lesions we investigated to what extent transgene expression could be regulated in accordance with the activation of the endogenous GFAP gene. In animals receiving excitotoxic lesions of the striatum we detected an eightfold increase of green fluorescent protein (GFP)-expressing cells. The vast majority of these cells did not divide, suggesting that the transgene was indeed regulated in a similar fashion as the endogenous GFAP gene. This finding will lead to the development of lentiviral vectors with autoregulatory capacities that may be very useful for gene therapy to the brain.

Publishing year

2004

Language

English

Pages

761-765

Publication/Series

European Journal of Neuroscience

Volume

19

Issue

3

Document type

Journal article

Publisher

Wiley-Blackwell

Topic

  • Neurosciences

Keywords

  • Human
  • Glial Fibrillary Acidic Protein: metabolism
  • Glial Fibrillary Acidic Protein: genetics
  • Genetic Vectors: metabolism
  • Female
  • Excitatory Amino Acid Agonists: toxicity
  • Enzyme-Linked Immunosorbent Assay
  • Comparative Study
  • Cell Count
  • Brain Injuries: virology
  • Brain Injuries: pathology
  • Brain Injuries: metabolism
  • Brain Injuries: chemically induced
  • Animals
  • Support
  • Sprague-Dawley
  • Transgenes
  • Non-U.S. Gov't
  • Gene Transfer Techniques
  • Gene Expression
  • Rats
  • Proliferating Cell Nuclear Antigen: genetics
  • Phosphopyruvate Hydratase: metabolism
  • Proliferating Cell Nuclear Antigen: metabolism
  • Luminescent Proteins: metabolism
  • Ibotenic Acid: toxicity
  • Lentivirus: genetics
  • Hydroxydopamines: toxicity

Status

Published

Research group

  • Molecular Neurogenetics
  • Neurobiology
  • CNS Gene Therapy

ISBN/ISSN/Other

  • ISSN: 1460-9568