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Cloning and characterization of cDNA encoding a novel human leukotriene B(4) receptor

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Author

Summary, in English

By homology screening using BLAST searches of expressed sequence tags (ESTs), we have found a previously unidentified cDNA encoding a putative seven-transmembrane receptor with highest similarity to the leukotriene B(4) receptor, BLTR. Analysis of calcium flow in transfected cells, along with sequence analysis, revealed that the EST encoded a functionally inactive protein, lacking the segment corresponding to the C-terminal part of the putative receptor protein. The missing segment was obtained by PCR amplification of a human leukocyte cDNA library and ligated to the truncated EST cDNA. The novel cDNA encodes a full-length receptor with 39% identity to the previously cloned BLTR. Studies of intracellular calcium flow of transfected HeLa cells exposed to various leukotrienes showed that also the novel BLTR-like receptor can be activated by leukotriene B(4), and it is therefore tentatively named BLTR2.

Department/s

Publishing year

2000

Language

English

Pages

377-382

Publication/Series

Biochemical and Biophysical Research Communications

Volume

274

Issue

2

Document type

Journal article

Publisher

Elsevier

Topic

  • Biological Sciences

Keywords

  • leukotriene B4
  • BLTR2
  • BLTR
  • calcium assay
  • 7TM receptor

Status

Published

Research group

  • Immunology
  • Drug Target Discovery

ISBN/ISSN/Other

  • ISSN: 1090-2104