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Flow-through microdispenser for interfacing mu-HPLC to Raman and mid-IR spectroscopic detection

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Author

Summary, in English

A flow-through microdispenser has been coupled to a micro HPLC separation system and used as a solvent elimination interface for Fourier transform infrared (FTIR) and Raman spectroscopic detection of the separated compounds. Using the microdispenser picoliter sized droplets can be generated and deposited on an appropriate target placed on a computerized x, y-stage. Evaporation of volatile solvent and buffer is rapid and allows analysis of the obtained dry deposits by various techniques. Due to the destruction free character of Raman and FTIR spectroscopy they can be applied sequentially to interrogate the same deposit. In the reported application five phenolic acids typically present in wine have been separated on a C-18 column technique using a mixture of water, methanol and acetic acid as mobile phase. For spectrum acquisition infrared and Raman microscopes have been used. The spectra recorded from the dried deposits of the separated compounds agreed well with the reference spectra of corresponding components.

Publishing year

2005

Language

English

Pages

132-139

Publication/Series

Journal of Chromatography A

Volume

1080

Issue

2

Document type

Journal article

Publisher

Elsevier

Topic

  • Medical Engineering

Keywords

  • flow through
  • HPLC
  • Raman microspectrometry
  • FTIR microscopy
  • microdispenser

Status

Published

ISBN/ISSN/Other

  • ISSN: 0021-9673