Effects of ethanol on muscarinic receptor-stimulated c-fos expression in human neuroblastoma cells
Author
Summary, in English
The effect of ethanol exposure on muscarinic receptor-stimulated expression of c-fos was investigated in SH-SY5Y cells. Four days of ethanol exposure enhanced carbachol-stimulated c-fos mRNA expression, analyzed with Northern blot, and Fos/AP-1 binding activity, measured with gel mobility super shift assay. Pre-incubation with muscarinic antagonists or the protein kinase C inhibitor GF109203X demonstrated that, in both control and ethanol-treated cells, carbachol-induced c-fos expression was mediated via muscarinic M1 receptors and to a large extent through protein kinase C. However, phorbol ester-induced c-fos expression was unaffected in ethanol-treated cells. Acute exposure to ethanol caused a suppression of both carbachol- and phorbol ester-stimulated c-fos expression. These results demonstrate that muscarinic receptor-stimulated gene expression is sensitive to both acute and long-term ethanol exposure.
Department/s
Publishing year
1997
Language
English
Pages
77-84
Publication/Series
Brain Research. Molecular Brain Research
Volume
46
Issue
1-2
Document type
Journal article
Publisher
Elsevier
Topic
- Medicinal Chemistry
- Clinical Medicine
- Cancer and Oncology
- Pharmacology and Toxicology
Keywords
- Muscarinic acetylcholine receptor
- Ethanol
- c-fos expression
- Protein kinase C
- Neuroblastoma SH-SY5Y cell
Status
Published
Research group
- Clinical Chemistry, Malmö
ISBN/ISSN/Other
- ISSN: 0169-328X