Ubiquinone-1 induces external deamino-NADH oxidation in potato tuber mitochondria
Author
Summary, in English
The addition of ubiquinone-1 (UQ-1) induced Ca2+-independent oxidation of deamino-NADH and NADH by intact potato (Solanum tuberosum L. cv Bintje) tuber mitochondria. The induced oxidation was coupled to the generation of a membrane potential. Measurements of NAD+-malate dehydrogenase activity indicated that the permeability of the inner mitochondrial membrane to NADH and deamino-NADH was not altered by the addition of UQ-1. We conclude that UQ-1-induced external deamino-NADH oxidation is due to a change in specificity of the external rotenone-insensitive NADH dehydrogenase. The addition of UQ-1 also induced rotenone-insensitive oxidation of deamino-NADH by inside-out submitochondrial particles, but whether this was due to a change in the specificity of the internal rotenone-insensitive NAD(P)H dehydrogenase or to a bypass in complex I could not be determined.
Department/s
Publishing year
1996-09
Language
English
Pages
75-78
Publication/Series
Plant Physiology
Volume
112
Issue
1
Links
Document type
Journal article
Publisher
American Society of Plant Biologists
Topic
- Biochemistry and Molecular Biology
Status
Published
Research group
- Plant Biology
ISBN/ISSN/Other
- ISSN: 0032-0889