Apolipoprotein M associates to lipoproteins through its retained signal peptide.

Summary, in English

Apolipoprotein M (apoM) is predominantly associated with HDL. In this study, it was investigated whether apoM's uncleaved signal peptide is necessary for the protein's ability to associate with lipoproteins. ApoM with a cleavable signal peptide, Q22A, was expressed, together with wild-type apoM, in HEK293 cells. On size-exclusion chromatography, the elution profile of wild-type apoM was similar to that of human HDL-associated plasma apoM. In contrast, the size of the Q22A mutant corresponded to free, unassociated apoM. This strongly indicates that the signal peptide is indeed necessary for apoM's ability to associate with lipid.

• Author: Olof Axler; Josefin Ahnström; Björn Dahlbäck
• Department/s: Clinical Chemistry, Malmö
• Publishing year: 2008
• Language: English
• Pages: 826-828
• Publication/Series: FEBS Letters
• Volume: 582
• Issue: 5
• Full text: - 137 kB; Download statistics
• Links: http://www.ncbi.nlm.nih.gov/pubmed/18279674?dopt=Abstract; http://dx.doi.org/10.1016/j.febslet.2008.02.007
• Document type: Journal article
• Publisher: Wiley-Blackwell
• Topic: Biological Sciences
• Status: Published
• Research group: Clinical Chemistry, Malmö
• ISBN/ISSN/Other: ISSN: 1873-3468