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Genomic structure of mouse SPI-C and genomic structure and expression pattern of human SPI-C.

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Author

  • Robert Carlsson
  • Anna Hjalmarsson
  • David Liberg
  • Christine Persson
  • Tomas Leanderson

Summary, in English

Erythroblast transformation-specific domain (ETS) transcription factors regulate some of the critical molecular mechanisms controlling the differentiation of multipotent haematopoietic progenitor cells into effector B-lymphocytes. The SPI-group ETS-protein transcription factors PU.1 and SPI-B play essential and, although coexpressed and binding to similar DNA sequences, unique roles in B-cell differentiation in mice. Mouse SPI-C is an SPI-group ETS protein expressed temporarily during B-cell development and in macrophages. Here we present the genomic organization of the mouse SPI-C gene, and show by rapid amplification of cDNA ends (5′-RACE) analysis that transcription of the mouse SPI-C mRNA starts at a single site producing a single processed transcript. We have also isolated a cDNA clone encoding the human SPI-C homologue, which displays 65% amino acid identity to the murine protein. In addition, we show that the genomic structure of the human and mouse genes are similar, containing a 5′ non-coding exon followed by five coding exons. Human SPI-C mRNA is preferentially detected in foetal and adult spleen, lymph nodes and at lower levels in bone marrow and foetal liver. Finally a phylogenetic prediction analysis of SPI-group protein sequences suggest that the SPI-C proteins form a distinct subgroup, with human SPI-C being closest related to the mouse SPI-C protein.

Department/s

Publishing year

2002

Language

English

Pages

271-278

Publication/Series

Gene

Volume

299

Issue

1-2

Document type

Journal article

Publisher

Elsevier

Topic

  • Immunology in the medical area

Keywords

  • Evolution
  • Erythroblast transformation specific domain
  • PU.1
  • Transcription
  • B cell
  • Exon

Status

Published

Research group

  • Immunology

ISBN/ISSN/Other

  • ISSN: 1879-0038