Isolation and characterisation of putative adhesins from Helicobacter pylori with affinity for heparan sulphate proteoglycan
Author
Summary, in English
A pool of heparan sulphate-binding proteins (HSBPs) from Helicobacter pylori culture supernates was obtained by sequential ammonium sulphate precipitation and affinity chromatography on heparin-Sepharose, The chromatographic procedure yielded one major fraction that contained proteins with heparan sulphate affinity as revealed by inhibition studies of heparan sulphate binding to H. pylori cells. Preparative iso-electric focusing, SDS-PACE and blotting experiments, with peroxidase(POD)-labelled heparan sulphate as a probe, indicated the presence of two major extracellular proteins with POD-heparan sulphate affinity. One protein had a molecular mass of 66.2 kDa and a pI of 5.4, whilst the second protein had a molecular mass of 71.5 kDa and a pI of 5.0. The N-terminal amino acid sequence of the 71.5-kDa HSBP did not show homology to any other heparin-binding protein, nor to known proteins of H, pylori, whereas the 66.2-kDa HSBP showed a high homology to an Escherichia coli chaperon protein and equine haemoglobin. A third HSBP was isolated from an outer-membrane protein (OMP) fraction of H. pylori cells with a molecular mass of 47.2 kDa, The amino acid sequence of an internal peptide of the OMP-HSBP did not show homology to the extracellular HSBP of H, pylori, or to another microbial HSBP.
Department/s
Publishing year
2001
Language
English
Pages
215-222
Publication/Series
Journal of Medical Microbiology
Volume
50
Issue
3
Links
Document type
Journal article
Publisher
Lippincott Williams & Wilkins
Topic
- Microbiology in the medical area
Status
Published
ISBN/ISSN/Other
- ISSN: 0022-2615