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A Probing Feeding Strategy for Escherichia coli Cultures

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Author

Summary, in English

A strain-independent feeding strategy for fed-batch cultures of <em>Escherichia coli</em> is presented. The key idea is to exploit a characteristic change in the cell metabolism at the onset of acetate formation. By superimposing short pulses in the glucose feed rate, on-line detection of acetate formation can be made using a standard dissolved oxygen sensor. A simple feedback algorithm is then used to adjust the feed rate to avoid acetate formation. The feasibility of the strategy is demonstrated by both simulation and experiments.

Publishing year

1999

Language

English

Pages

523-528

Publication/Series

Biotechnology Techniques

Volume

13

Document type

Journal article

Publisher

Springer

Topic

  • Control Engineering

Keywords

  • Escherichia coli
  • fed-batch culture
  • glucose
  • acetate
  • feedback control
  • dissolved oxygen.

Status

Published

ISBN/ISSN/Other

  • ISSN: 0951-208X