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Growth kinetics and Pho84 phosphate transporter activity of Saccharomyces cerevisiae under phosphate-limited conditions

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Author

  • Soheila Shokrollahzadeh
  • Babak Bonakdarpour
  • Farzaneh Vahabzadeh
  • Mehri Sanati

Summary, in English

The effect of phosphate (P ( i )) concentration on the growth behavior of Saccharomyces cerevisiae strain CEN.PK113-5D in phosphate-limited batch and chemostat cultures was studied. The range of dilution rates used in the present study was 0.08-0.45 h(-1). The batch growth of yeast cells followed Monod relationship, but growth of the cells in phosphate-limited chemostat showed change in growth kinetics with increasing dilution rates. The difference in growth kinetics of the yeast cells in phosphate-limited chemostat for dilution rates below and above approximately 0.2 h(-1) has been discussed in terms of the batch growth kinetic data and the change in the metabolic activity of the yeast cells. Immunological detection of a C-terminally myc epitope-tagged Pho84 fusion protein indicated derepressive expression of the Pho84 high-affinity P ( i ) transporter in the entire range of dilution rates employed in this study. Phosphate transport activity mediated by Pho84 transporter was highest at very low dilution rates, i.e. 0.08-0.1 h(-1), corresponding to conditions in which the amount of synthesized Pho84 was at its maximum.

Publishing year

2007

Language

English

Pages

17-25

Publication/Series

Journal of Industrial Microbiology & Biotechnology

Volume

34

Issue

1

Document type

Journal article

Publisher

Springer

Topic

  • Production Engineering, Human Work Science and Ergonomics

Keywords

  • phosphate
  • Pho84 transporter
  • growth kinetics
  • saccharomyces cerevisiae

Status

Published

ISBN/ISSN/Other

  • ISSN: 1367-5435