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Building a map of the breast cancer proteome - Strategies to increase coverage

På svenska

Author

  • Paolo Cifani

Summary, in English

Amongst the various –omics sciences, proteomics has the highest potential for functional characterization and consequently can contribute significantly to the field of cancer research. In particular, the focus of this thesis is on breast cancer. Alas, since state-of-the-art technologies cannot meet the complexity of upper eukaryotic proteomes, a complete resolution of clinical samples is still unachievable. Comprehensive mapping of proteins involved in cancer and of their PTMs is proposed in this thesis as a general strategy to increase the output of mass-spectrometry based proteomics. Different approaches to improve the coverage of this map are proposed: optimization of sample fractionation, focusing on difficult sub-proteomes, targeting of specific biological processes and optimization of data analysis. A combination of these approaches will provide a growing collection of empirical MS-spectra, which will enhance the detection by shotgun proteomics and facilitate the transition towards the development of targeted assays.

Publishing year

2013

Language

English

Document type

Dissertation

Topic

  • Immunology in the medical area

Keywords

  • Quantitative proteomics
  • Mass-Spectrometry
  • Cancer
  • DNA repair
  • Hypoxia
  • PTMs
  • Sample fractionation
  • Database

Status

Published

Supervisor

ISBN/ISSN/Other

  • ISBN: 978-7473-529-1

Defence date

31 May 2013

Defence time

09:15

Defence place

Lecture Hall, Medical Village, Scheelevägen 2, Lund

Opponent

  • Luca Bini