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Purification and functional characterization of factor I.

På svenska

Author

Summary, in English

Factor I (FI) is a soluble, 88 kDa glycoprotein present in plasma at a concentration of approximately 35 mg/L. FI inhibits all complement pathways as it degrades activated C4b and C3b when these are bound to a cofactor such as C4b-binding protein or factor H. Here, we describe a method for purification of FI from human plasma, which is based on affinity chromatography followed by anion exchange chromatography. We also describe a functional assay, in which activity of FI can be assessed.

Publishing year

2014

Language

English

Pages

177-188

Publication/Series

Methods in Molecular Biology

Volume

1100

Document type

Journal article

Publisher

Springer

Topic

  • Other Basic Medicine

Status

Published

Research group

  • Protein Chemistry, Malmö

ISBN/ISSN/Other

  • ISSN: 1940-6029