Characterization of the biochemical and biophysical properties of the Saccharomyces cerevisiae phosphate transporter Pho89
Author
Summary, in English
In Saccharomyces cerevisiae, Pho89 mediates a cation-dependent transport of Pi across the plasma membrane. This integral membrane protein belongs to the Inorganic Phosphate Transporter (PiT) family, a group that includes the mammalian Na(+)/Pi cotransporters Pit1 and Pit2. Here we report that the Pichia pastoris expressed recombinant Pho89 was purified in the presence of Foscholine-12 and functionally reconstituted into proteoliposomes with a similar substrate specificity as observed in an intact cell system. The alpha-helical content of the Pho89 protein was estimated to 44%. EPR analysis showed that purified Pho89 protein undergoes conformational change upon addition of substrate.
Department/s
Publishing year
2013-07-05
Language
English
Pages
551-556
Publication/Series
Biochemical and Biophysical Research Communications
Volume
436
Issue
3
Document type
Journal article
Publisher
Elsevier
Topic
- Biological Sciences
Keywords
- Pho89
- Pichia pastoris
- Oligomer
- Reconstitution
- Phosphate transport
- Circular dichroism
Status
Published
ISBN/ISSN/Other
- ISSN: 1090-2104