Large-scale inference of protein tissue origin in gram-positive sepsis plasma using quantitative targeted proteomics.
Author
Summary, in English
The plasma proteome is highly dynamic and variable, composed of proteins derived from surrounding tissues and cells. To investigate the complex processes that control the composition of the plasma proteome, we developed a mass spectrometry-based proteomics strategy to infer the origin of proteins detected in murine plasma. The strategy relies on the construction of a comprehensive protein tissue atlas from cells and highly vascularized organs using shotgun mass spectrometry. The protein tissue atlas was transformed to a spectral library for highly reproducible quantification of tissue-specific proteins directly in plasma using SWATH-like data-independent mass spectrometry analysis. We show that the method can determine drastic changes of tissue-specific protein profiles in blood plasma from mouse animal models with sepsis. The strategy can be extended to several other species advancing our understanding of the complex processes that contribute to the plasma proteome dynamics.
Department/s
Publishing year
2016
Language
English
Publication/Series
Nature Communications
Volume
7
Full text
Links
Document type
Journal article
Publisher
Nature Publishing Group
Topic
- Cell and Molecular Biology
Status
Published
Project
- Proteomic profiling of bacterial host adaptation - Racing the Red Queen
Research group
- Infection Medicine Proteomics
- SEBRA Sepsis and Bacterial Resistance Alliance
- epIgG
ISBN/ISSN/Other
- ISSN: 2041-1723