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Inflammatory Markers in Huntington’s Disease Plasma – A Robust NanoLC-MRM-MS Assay Development

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Author

Summary, in English

The development of a quantitative multiplex reaction monitoring (MRM) assay is presented for the measurements of seven inflammatory markers in human plasma, using nanoLC-MS/MS analysis with stable isotope dilution strategy. We report a robust and sensitive, mass spectrometry based, quantitative assay with a relative standard deviation of <15% that accounts for the entire sample processing, while the analytical reproducibility of the assay was 3.6%. We used plasma from Huntington´s disease (HD) gene carriers and healthy controls to compare our MRM method with established antibody based methods. Interestingly, we found a good agreement between assays for the measurement of C-reactive protein (CRP).

Publishing year

2014

Language

English

Pages

68-75

Publication/Series

EuPA Open Proteomics

Volume

3

Document type

Journal article

Publisher

Elsevier

Topic

  • Medical Engineering

Keywords

  • multiple reaction monitoring
  • complement components
  • C-reactive protein
  • Huntington´s disease

Status

Published

Research group

  • Biomarkers in Brain Disease

ISBN/ISSN/Other

  • ISSN: 2212-9685