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Novel anti-apoptotic effect of Bcl-2: Prevention of polyamine depletion-induced cell death

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Author

Summary, in English

The spermine analogue N(1),N(11)-diethylnorspermine (DENSPM) efficiently depletes the polyamine pools in the breast cancer cell line L56Br-C1 and induces apoptotic cell death via the mitochondrial pathway. In this study, we have over-expressed the anti-apoptotic protein Bcl-2 in L56Br-C1 cells and investigated the effect of DENSPM treatment. DENSPM-induced cell death was significantly reduced in Bcl-2 over-expressing cells. Bcl-2 over-expression reduced DENSPM-induced release of the pro-apoptotic proteins AIF, cytochrome c, and Smac/DIABLO from the mitochondria. Bcl-2 over-expression reduced the DENSPM-induced activation of caspase-3. Bcl-2 over-expression also prevented DENSPM-induced Bax cleavage and reduction of Bcl-X(L) and survivin levels. The DENSPM-induced activation of the polyamine catabolic enzyme spermidine/spermine N(1)-acetyltransferase was reduced by Bcl-2 over-expression, partly preventing polyamine depletion. Thus, Bcl-2 over-expression prevented a number of DENSPM-induced apoptotic effects

Publishing year

2008

Language

English

Pages

66-74

Publication/Series

Cell Biology International

Volume

32

Document type

Journal article

Publisher

Elsevier

Topic

  • Zoology

Keywords

  • Apoptosis
  • Spermine analogue
  • Cytochrome c
  • Human breast cancer
  • Bcl-2

Status

Published

ISBN/ISSN/Other

  • ISSN: 1095-8355