Comparing Analysis Methods in Functional Calcium Imaging of the Insect Brain.

Summary, in English

We investigate four different methods for background estimation in calcium imaging of the insect brain and evaluate their performance on six data sets consisting of data recorded from two sites in two species of moths. The calcium fluorescence decay curve outside the potential response is estimated using either a low-pass filter or constant, linear or polynomial regression, and is subsequently used to calculate the magnitude, latency and duration of the response. The magnitude and variance of the responses that are obtained by the different methods are compared, and, by computing the receiver operating characteristics of a classifier based on response magnitude, we evaluate the ability of each method to detect the stimulus type and conclude that a polynomial approximation of the background gives the overall best result.

• Author: Anna Balkenius; Anders J Johansson; Christian Balkenius
• Department/s: Functional zoology; Neuronano Research Center (NRC); Department of Electrical and Information Technology; Cognitive Science; Lund Vision Group
• Publishing year: 2015
• Language: English
• Publication/Series: PLoS ONE
• Volume: 10
• Issue: 6
• Links: http://www.ncbi.nlm.nih.gov/pubmed/26046538?dopt=Abstract; http://dx.doi.org/10.1371/journal.pone.0129614
• Document type: Journal article
• Publisher: Public Library of Science (PLoS)
• Topic: Neurosciences
• Status: Published
• Research group: Neuronano Research Center (NRC); Lund Vision Group
• ISBN/ISSN/Other: ISSN: 1932-6203