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The C-terminus of dUTPase: observation on flexibility using NMR

På svenska

Author

  • Johan Nord
  • Per-Olof Nyman
  • Gunilla Larsson
  • Torbjörn Drakenberg

Summary, in English

The dynamics of the C-terminus of the dUTPases from Escherichia coli and equine infectious anaemia virus (EIAV) were studied by 1H-15N nuclear magnetic resonance spectroscopy. The two enzymes differ with regard to flexibility in the backbone of the 15 most C-terminal amino acid residues, some of which are conserved and essential for enzymic activity. In the bacterial enzyme, the residues closest to the C-terminus are highly flexible and display a correlation time in the nanosecond time range. No similar high flexibility could be detected for the C-terminal part of EIAV dUTPase, indicating a different time range of flexibility.

Publishing year

2001

Language

English

Pages

228-232

Publication/Series

FEBS Letters

Volume

492

Issue

3

Document type

Journal article

Publisher

Wiley-Blackwell

Topic

  • Biological Sciences

Keywords

  • dUTPase
  • Equine infectious anemia virus
  • Flexible C-terminus
  • Nuclear magnetic resonance
  • Nucleotide binding
  • Escherichia coli

Status

Published

ISBN/ISSN/Other

  • ISSN: 1873-3468