Antibodies reactive to cleaved sites in complement proteins enable highly specific measurement of soluble markers of complement activation.

Summary, in English

An emerging number of diseases and therapeutic approaches with defined involvement of the complement system justify a need for specific markers reflecting activation of particular effector arms of the complement cascade. Measurement of such soluble markers in circulation is a challenge since the specificity of antibodies must be limited to activated complement fragments but not predominant and ubiquitous parental molecules. Existing assays for the measurement of soluble, activated complement proteins are based on the detection of conformational neoepitopes. We tested an alternative approach based on detection of short linear neoepitopes exposed at the cleavage sites after activation of the actual complement component. Obtained antibodies reactive to C4d and C5b fragments enabled us to set up highly specific sandwich ELISAs, which ensured trustful measurements without false positive readouts characteristic for some of the widely used assays.

• Author: Anna Blom; Anders Österborg; Tom E Mollnes; Marcin Okroj
• Department/s: BioCARE: Biomarkers in Cancer Medicine improving Health Care, Education and Innovation; Protein Chemistry, Malmö
• Publishing year: 2015
• Language: English
• Pages: 164-170
• Publication/Series: Molecular Immunology
• Volume: 66
• Issue: 2
• Full text: - 837 kB; Download statistics
• Links: http://www.ncbi.nlm.nih.gov/pubmed/25795308?dopt=Abstract; http://dx.doi.org/10.1016/j.molimm.2015.02.029
• Document type: Journal article
• Publisher: Pergamon Press Ltd.
• Topic: Immunology in the medical area
• Status: Published
• Research group: Protein Chemistry, Malmö
• ISBN/ISSN/Other: ISSN: 1872-9142