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BID Mediates Oxygen-Glucose Deprivation-Induced Neuronal Injury in Organotypic Hippocampal Slice Cultures and Modulates Tissue Inflammation in a Transient Focal Cerebral Ischemia Model without Changing Lesion Volume.

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Author

  • Nellie Anne Martin
  • Helena Bonner
  • Maria Louise Elkjær
  • Beatrice D'Orsi
  • Gang Chen
  • Hans Georg König
  • Martina Svensson
  • Tomas Deierborg
  • Shona Pfeiffer
  • Jochen H Prehn
  • Kate Lykke Lambertsen
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Summary, in English

The BH3 interacting-domain death agonist (BID) is a pro-apoptotic protein involved in death receptor-induced and mitochondria-mediated apoptosis. Recently, it has also been suggested that BID is involved in the regulation of inflammatory responses in the central nervous system. We found that BID deficiency protected organotypic hippocampal slice cultures in vitro from neuronal injury induced by oxygen-glucose deprivation. In vivo, BID-knockout (KO) mice and wild type (WT) mice were subjected to 60 min of transient middle cerebral artery occlusion (tMCAO) to induce focal cerebral ischemia, and allowed to recover for 24 h. Infarct volumes and functional outcome were assessed and the inflammatory response was evaluated using immunofluorescence, Western blotting, quantitative PCR (qPCR) and Mesoscale multiplex analysis. We observed no difference in the infarct volume or neurological outcome between BID-KO and WT mice. The inflammatory response was reduced by BID deficiency as indicated by a change in microglial/leukocyte response. In conclusion, our data suggest that BID deficiency is neuroprotective in an in vitro model and modulates the inflammatory response to focal cerebral ischemia in vivo. However, this is not translated into a robust neuroprotection in vivo.

Publishing year

2016

Language

English

Publication/Series

Frontiers in Cellular Neuroscience

Volume

10

Document type

Journal article

Publisher

Frontiers Media S. A.

Topic

  • Neurosciences

Status

Published

Research group

  • Neuroinflammation

ISBN/ISSN/Other

  • ISSN: 1662-5102