Ubiquitous Structural Signaling in Bacterial Phytochromes.
Author
Summary, in English
The phytochrome family of light-switchable proteins has long been studied by biochemical, spectroscopic and crystallographic means, while a direct probe for global conformational signal propagation has been lacking. Using solution X-ray scattering, we find that the photosensory cores of several bacterial phytochromes undergo similar large-scale structural changes upon red-light excitation. The data establish that phytochromes with ordinary and inverted photocycles share a structural signaling mechanism and that a particular conserved histidine, previously proposed to be involved in signal propagation, in fact tunes photoresponse.
Department/s
Publishing year
2015
Language
English
Pages
3379-3383
Publication/Series
The Journal of Physical Chemistry Letters
Volume
6
Issue
17
Document type
Journal article
Publisher
The American Chemical Society (ACS)
Topic
- Biochemistry and Molecular Biology
Status
Published
ISBN/ISSN/Other
- ISSN: 1948-7185