Proteome-wide selected reaction monitoring assays for the human pathogen Streptococcus pyogenes.
Author
Summary, in English
Selected reaction monitoring mass spectrometry (SRM-MS) is a targeted proteomics technology used to identify and quantify proteins with high sensitivity, specificity and high reproducibility. Execution of SRM-MS relies on protein-specific SRM assays, a set of experimental parameters that requires considerable effort to develop. Here we present a proteome-wide SRM assay repository for the gram-positive human pathogen group A Streptococcus. Using a multi-layered approach we generated SRM assays for 10,412 distinct group A Streptococcus peptides followed by extensive testing of the selected reaction monitoring assays in >200 different group A Streptococcus protein pools. Based on the number of SRM assay observations we created a rule-based selected reaction monitoring assay-scoring model to select the most suitable assays per protein for a given cellular compartment and bacterial state. The resource described here represents an important tool for deciphering the group A Streptococcus proteome using selected reaction monitoring and we anticipate that concepts described here can be extended to other pathogens.
Department/s
Publishing year
2012
Language
English
Publication/Series
Nature Communications
Volume
3
Full text
Links
Document type
Journal article
Publisher
Nature Publishing Group
Topic
- Infectious Medicine
Status
Published
Research group
- epIgG
- Infection Medicine Proteomics
- SEBRA Sepsis and Bacterial Resistance Alliance
ISBN/ISSN/Other
- ISSN: 2041-1723