Polyamines inhibit myosin phosphatase and increase LC20 phosphorylation and force in smooth muscle
Author
Summary, in English
The increase in Ca(2+)-activated force caused by polyamines in beta-escin-permeabilized guinda pig ileum is shown to be associated with increased myosin 20-kDa light chain (LC20) phosphorylation and shortening velocity. Myosin LC20 dephosphorylation with arrested kinase activity was slower in the presence of 1 mM spermine. Smooth muscle phosphatases (SMP-I, -II, -III, and -IV) isolated from turkey gizzard are all active against phosphorylated LC20, but only SMP-III and -IV dephosphorylate heavy meromyosin (HMM). Spermine inhibited SMP-III activity toward LC20 but stimulated HMM dephosphorylation, whereas SMP-IV was inhibited with both substrates. In contrast, SMP-I and -II were stimulated by spermine. The relative effects of different polyamines correlated with an increasing number of positive charges. Spermine did not affect binding of SMP-IV to myosin and did not dissociate any of the subunits of the enzyme. Incubation of permeabilized strips with SMP-IV resulted in attenuated responses to Ca2+, an effect that was opposed by spermine and abolished by microcystin-LR. We conclude that spermine selectively inhibits myosin phosphatase activity and suggest that polyamines function as endogenous myosin phosphatase inhibitors.
Department/s
Publishing year
1995-09
Language
English
Pages
563-571
Publication/Series
American Journal of Physiology: Cell Physiology
Volume
269
Issue
3
Links
Document type
Journal article
Publisher
American Physiological Society
Topic
- Physiology
Keywords
- Animals
- Female
- Gizzard
- Guinea Pigs
- Ileum
- In Vitro Techniques
- Muscle Contraction
- Muscle, Smooth
- Myosin Light Chains
- Myosin-Light-Chain Kinase
- Myosin-Light-Chain Phosphatase
- Permeability
- Phosphoprotein Phosphatases
- Phosphoric Monoester Hydrolases
- Phosphorylation
- Polyamines
- Spermine
- Turkeys
Status
Published
Research group
- Vascular Physiology
ISBN/ISSN/Other
- ISSN: 1522-1563