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Multicenter comparison trial of DNA extraction methods and PCR assays for detection of Chlamydia pneumoniae in endarterectomy specimens

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Author

  • Petra Apfalter
  • Francesco Blasi
  • Jens Boman
  • Charlotte A. Gaydos
  • Michael Kundi
  • Matthias Maass
  • Athanasios Makristathis
  • Adam Meijer
  • Reinhard Nadrchal
  • Kenneth Persson
  • Manfred L. Rotter
  • C. Y. William Tong
  • Gerold Stanek
  • Alexander M. Hirschl
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Summary, in English

The reported rate of detection of Chlamydia pneumoniae DNA within atherosclerotic lesions by PCR varies between 0 and 100%. In this study, identical sets of coded experimental atheroma samples (n = 15) and spiked controls (n = 5) were analyzed by 16 test methods in nine centers by means of PCR. The positive controls were correctly identified to levels of 1, 0.1, and 0.01 inclusion bodies of C. pneumoniae/ml of tissue homogenate by 16 (100%), 11 (69%), and 3 (19%) of the test methods, respectively. Three out of 16 negative controls (19%) were rated positive. Positivity rates for atheroma samples varied between 0 and 60% for the different test methods, with the maximum concordant result for positivity being only 25% for one carotid artery sample. There was no consistent pattern of positive results among the various laboratories, and there was no correlation between the detection rates and the sensitivity of the assay used.

Publishing year

2001

Language

English

Pages

519-524

Publication/Series

Journal of Clinical Microbiology

Volume

39

Issue

2

Document type

Journal article

Publisher

American Society for Microbiology

Topic

  • Microbiology in the medical area

Status

Published

Research group

  • Clinical Microbiology, Malmö

ISBN/ISSN/Other

  • ISSN: 1098-660X