Monitoring of enzymatic hydrolysis of starch by microdialysis sampling coupled on-line to anion exchange chromatography and integrated pulsed electrochemical detection using post-column switching

Summary, in English

A quantitative evaluation of the hydrolysis of wheat starch using Termamyl, a thermostable alpha-amylase (endo-l,4-alpha-D-glucan, glucanohydrolase; EC 3.2.1.78), is reported. Data from the monitoring of the hydrolysis of wheat starch indicated that, after 1 h, glucose and maltooligosaccharides up to DP 7 were the main hydrolysis products and thus enabled optimization of a liquefication step during the production of L-lactic acid. The monitoring system used, both in the on- and off-line mode, was based on continuous flow microdialysis sampling (CFMS) coupled to anion exchange chromatography and integrated pulsed electrochemical detection (IPED). A microdialysis probe equipped with a 5-mm polysulfone (SPS 4005) membrane, with a molecular-weight cut-off of 5 kDa, was used to sample the hydrolysis products of native wheat starch at 90 degrees C. Characteristic fingerprint separations were achieved by anion exchange chromatography after enzymatic hydrolysis. Post-column switching improved the detection and, consequently, also quantification of the hydrolysates as fouling of the electrode could be reduced. Maltooligosaccharide standards were used for quantification and to verify the elution of the hydrolysates by spiking the off-line samples. (C) 1997 John Wiley & Sons, Inc.

• Author: N Torto; Lo Gorton; György Marko-Varga; Jenny Emnéus; C Akerberg; Guido Zacchi; Thomas Laurell
• Department/s: Centre for Analysis and Synthesis; Department of Biomedical Engineering; Division of Chemical Engineering
• Publishing year: 1997
• Language: English
• Pages: 546-554
• Publication/Series: Biotechnology and Bioengineering
• Volume: 56
• Issue: 5
• Links: http://dx.doi.org/10.1002/(SICI)1097-0290(19971205)56:5<546::AID-BIT8>3.0.CO;2-I
• Document type: Journal article
• Publisher: John Wiley & Sons Inc.
• Topic: Analytical Chemistry
• Keywords: post-column switching; microdialysis; monitoring; hydrolysates; integrated pulsed electrochemical detection
• Status: Published
• ISBN/ISSN/Other: ISSN: 1097-0290