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Global and 3D Spatial Assessment of Neuroinflammation in Rodent Models of Multiple Sclerosis.

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Author

  • Shashank Gupta
  • Regine Utoft
  • Henrik Hasseldam
  • Anja Schmidt-Christensen
  • Tine Hannibal
  • Lisbeth Hansen
  • Nina Fransén Pettersson
  • Noopur Agarwal-Gupta
  • Björn Rozell
  • Åsa Andersson
  • Dan Holmberg
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Summary, in English

Multiple Sclerosis (MS) is a progressive autoimmune inflammatory and demyelinating disease of the central nervous system (CNS). T cells play a key role in the progression of neuroinflammation in MS and also in the experimental autoimmune encephalomyelitis (EAE) animal models for the disease. A technology for quantitative and 3 dimensional (3D) spatial assessment of inflammation in this and other CNS inflammatory conditions is much needed. Here we present a procedure for 3D spatial assessment and global quantification of the development of neuroinflammation based on Optical Projection Tomography (OPT). Applying this approach to the analysis of rodent models of MS, we provide global quantitative data of the major inflammatory component as a function of the clinical course. Our data demonstrates a strong correlation between the development and progression of neuroinflammation and clinical disease in several mouse and a rat model of MS refining the information regarding the spatial dynamics of the inflammatory component in EAE. This method provides a powerful tool to investigate the effect of environmental and genetic forces and for assessing the therapeutic effects of drug therapy in animal models of MS and other neuroinflammatory/neurodegenerative disorders.

Department/s

Publishing year

2013

Language

English

Publication/Series

PLoS ONE

Volume

8

Issue

10

Document type

Journal article

Publisher

Public Library of Science (PLoS)

Topic

  • Basic Medicine

Status

Published

Project

  • 3D imaging of inflammation and autoimmunity

Research group

  • Autoimmunity
  • Immunology

ISBN/ISSN/Other

  • ISSN: 1932-6203