Molecular microheterogeneity of prostate specific antigen in seminal fluid by mass spectrometry.
Author
Summary, in English
OBJECTIVES: Prostate specific antigen (PSA) is a widely used and clinically valuable marker for prostate disease. In order to enable the development of new PSA assays and progress the understanding of the biology of PSA we have analyzed PSA in seminal plasma. Design and methods PSA in seminal plasma from men attending a fertility clinic and healthy controls was analyzed using SDS-PAGE, Western blotting and mass spectrometry. RESULTS: Using mass spectrometry, different forms of PSA could be identified in 1-9 bands seen on SDS-PAGE analysis of the respective sample. However, a majority of these molecular forms of PSA were not observed on Western blots. Enzymatic activity of PSA isoforms was demonstrated by sequencing data in zymogram gels. Multivariate analysis of clinical data revealed well-separated patient groups. CONCLUSIONS: We demonstrated that PSA in seminal plasma occurs in several isoforms, yet not all were detectable using an antibody based clinical routine method. The heterogeneity of PSA expression might be of clinical significance, by an improved patient phenotyping.
Department/s
- Department of Biomedical Engineering
- Breastcancer-genetics
- Clinical Chemistry, Malmö
- EpiHealth: Epidemiology for Health
- MultiPark: Multidisciplinary research focused on Parkinson´s disease
- BioCARE: Biomarkers in Cancer Medicine improving Health Care, Education and Innovation
Publishing year
2012
Language
English
Pages
331-338
Publication/Series
Clinical Biochemistry
Volume
45
Issue
4-5
Full text
Links
Document type
Journal article
Publisher
Elsevier
Topic
- Biochemistry and Molecular Biology
Keywords
- ESI-LTQ FT-ICR
- MALDI LTQ Orbitrap XL
- infertility
- seminal plasma
- prostate specific antigen
- isoform
Status
Published
Research group
- Clinical Chemistry, Malmö
ISBN/ISSN/Other
- ISSN: 1873-2933