First attempt to produce experimental Campylobacter concisus infection in mice
Author
Summary, in English
AIM: To infect mice with atypical Campylobacter concisus (C concisus) for the first time. METHODS: Three separate experiments were conducted in order to screen the ability of five clinical C concisus isolates of intestinal origin and the ATCC 33237 type strain of oral origin to colonize and produce infection in immunocompetent BALB/cA mice. The majority of the BALB/cA mice were treated with cyclophosphamide prior to C concisus inoculation to suppress immune functions. Inoculation of C concisus was performed by the gastric route. RESULTS: C concisus was isolated from the liver, ileum and jejunum of cyclophosphamide-treated mice in the first experiment. No C concisus strains were isolated in the two subsequent experiments. Mice infected with C concisus showed a significant loss of body weight from day two through to day five of infection but this decreased at the end of the first week. Histopathologicalexamination did not consistently find signs of inflammation in the gut, but occasionally microabscesses were found in the liver of infected animals. CONCLUSION: Transient colonization with C concisus was observed in mice with loss of body weight. Future studies should concentrate on the first few days after inoculation and in other strains of mice. (C) 2008 The WJG Press. All rights reserved.
Publishing year
2008
Language
English
Pages
6954-6959
Publication/Series
World Journal of Gastroenterology
Volume
14
Issue
45
Document type
Journal article
Publisher
WJG Press
Topic
- Gastroenterology and Hepatology
Keywords
- Campylobacter concisus
- Animal model
- BALB/cA mice
- Infection
- Colonization
Status
Published
ISBN/ISSN/Other
- ISSN: 1007-9327