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Immunogenic proteins of Helicobacter pullorum, Helicobacter bilis and Helicobacter hepaticus identified by two-dimensional gel electrophoresis and immunoblotting.

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Author

  • Iryna Kornilovska
  • Ingrid Nilsson
  • Meeme Utt
  • Åsa Ljungh
  • Torkel Wadström

Summary, in English

The ecological niches occupied by various species of Helicobacter are not yet known and the full spectrum of diseases associated with Helicobacter infections are not yet defined. Since these fastidious microaerofilic bacteria require special growth conditions new and improved molecular and serologic diagnostic methods have been developed to increase our understanding of their pathogenesis and virulence characteristics. Immunogenic cell surface proteins of Helicobacter pullorum, Helicobacter bilis, and Helicobacter hepaticus were characterised by proteomic techniques using two-dimensional electrophoresis and immunoblotting with antisera from immunised rabbits. Cross-reactivity between the three Helicobacter species were analysed after a four-step cross-absorption experiment. For H. pullorum, H. bilis and H. hepaticus 21, 13 and 27 specific immunogenic proteins, respectively, were identified. These proteins could be of important sero-diagnostic value for analyses of sera from humans, laboratory animals and for the veterinarian field.

Publishing year

2002

Language

English

Pages

775-783

Publication/Series

Proteomics

Volume

2

Issue

6

Document type

Journal article

Publisher

John Wiley & Sons Inc.

Topic

  • Microbiology in the medical area

Status

Published

Research group

  • Celiac Disease and Diabetes Unit

ISBN/ISSN/Other

  • ISSN: 1615-9861