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Radioimmunoassay of beta-microseminoprotein, a prostatic-secreted protein present in sera of both men and women

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Author

Summary, in English

We describe a simple radioimmunoassay of beta-microseminoprotein, one of the three most abundant secretory proteins of the prostate gland. The detection limit of the assay is 1 microgram/L, and its precision, expressed as the total coefficient of variation, is less than 10% for values between 10 and 150 micrograms/L. Using this assay, we found that beta-microseminoprotein immunoreactivity was present in sera from both sexes at about the same concentration. The protein detected had the same molecular size on gel chromatography as the protein isolated from seminal plasma, and dilution curves for the sera paralleled that for the pure protein. The findings suggest that beta-microseminoprotein is present in serum of healthy subjects of both sexes and that it originates in tissue other than the prostate gland. The range of the serum concentration was 0-10.6 micrograms/L (median 4.1) for 51 healthy adult women and 1.1-14.7 micrograms/L (median 6.2) for 35 healthy adult men not older than 40 years. In males with prostatic cancer the concentration in serum was highly variable and often greatly increased. The concentration of beta-microseminoprotein was correlated with that of creatinine in serum, suggesting that the protein is eliminated--at least partly--from the circulation by glomerular filtration. Little of the protein was present in the urine of women. In urine from men the concentration was high and variable, probably because of local contribution from the prostate gland to the urethral urine.

Publishing year

1989

Language

English

Pages

1497-1503

Publication/Series

Clinical Chemistry

Volume

35

Issue

7

Document type

Journal article

Publisher

American Association for Clinical Chemistry

Topic

  • Biochemistry and Molecular Biology

Keywords

  • fertility
  • gonadotropins
  • beta-inhibin
  • prostatic cancer
  • reference values
  • renal function
  • seminal plasma
  • urine

Status

Published

Research group

  • Urological cancer, Malmö
  • Clinical Chemistry, Malmö

ISBN/ISSN/Other

  • ISSN: 0009-9147