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Oligosaccharide mapping of proteoglycan-bound and xyloside-initiated dermatan sulfate from fibroblasts

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Author

Summary, in English

The copolymeric structure of dermatan sulfate chains synthesized by skin fibroblasts has been examined. Chains initiated onto exogeneous p-nitrophenyl-beta-D-xylopyranoside or attached to protein in a large proteoglycan, PG-L, and two small proteoglycans, PG-S1 and PG-S2, have been compared by using high resolution electrophoresis and gel chromatography of oligosaccharides generated by specific enzymatic or chemical degradations. The results confirm that chains attached to PG-L are glucuronate-rich, whereas novel findings indicate that chains attached to either of the two PG-S variants yield closely similar oligosaccharide maps, have approximately equal glucuronate and iduronate content and contain over 90% 4-sulfated disaccharide repeating units. Dermatan sulfate chains built onto xyloside at concentrations of 50 microM and below have a copolymeric structure similar to that of chains from the two PG-S variants. These findings indicate that the polymer-modifying machinery can generate chains with extended iduronate-containing repeats also when the xylose primer is not linked to core protein.

Publishing year

1991

Language

English

Pages

108-115

Publication/Series

Glycoconjugate Journal

Volume

8

Issue

2

Document type

Journal article

Publisher

Springer

Topic

  • Medicinal Chemistry
  • Cell and Molecular Biology
  • Dermatology and Venereal Diseases

Keywords

  • oligosaccharide mapping
  • xylosides
  • dermatan sulfate
  • Proteoglycans

Status

Published

Research group

  • Glycobiology
  • Matrix Biology

ISBN/ISSN/Other

  • ISSN: 1573-4986