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Separation and surveys of proteins of Helicobacter pylori.

På svenska

Author

  • Ingrid Nilsson
  • Meeme Utt

Summary, in English

The analysis of Helicobacter pylori proteins is a demanding task for the elucidation of virulence factors, antigens and vaccines, all important for diagnosis, therapy and protection. In the "pre-genomic era" the purification of proteins was mostly performed by using various techniques such as detergent treatment of the bacterial cells, ultra-centrifugation, various chromatographic methods, antibody detection, N-terminal sequence determination and finally cloning and identification of the corresponding gene. In this review, the most representative methods used for purification, separation and identification of H. pylori proteins will be presented as well as some important developments in the "post-genomic era" that have improved the performance of these characterisation techniques.

Publishing year

2002

Language

English

Pages

251-260

Publication/Series

Journal of Chromatography. B

Volume

771

Issue

1-2

Document type

Journal article review

Publisher

Elsevier

Topic

  • Microbiology in the medical area

Keywords

  • Helicobacter pylori : chemistry
  • Bacterial
  • Genome
  • Two-Dimensional
  • Gel
  • Electrophoresis
  • Bacterial Proteins : isolation & purification
  • Bacterial Proteins : genetics
  • Proteome
  • Helicobacter pylori : genetics
  • Helicobacter pylori : pathogenicity

Status

Published

Research group

  • Celiac Disease and Diabetes Unit

ISBN/ISSN/Other

  • ISSN: 1873-376X